Abstract
We report a rapid and highly versatile one-step single tube method for gene amplification and detection using a non-radiolabelled probe. The technique requires nylon on which a third primer has been immobilized that acts as a probe and digoxigenin-labelled-dUTP added to the conventional PCR mixture. After PCR amplification the membrane-impregnated probe is labelled with dig-11-dUTP that serves to confirm the identity of the PCR product.
